Gefei Li, Wenxiao Ma, Juan Mo, Boyang Cheng, Shin-ichiro Shoda, Demin Zhou*, and Xin-Shan Ye*，Influenza Virus Precision Diagnosis and Continuous Purification Enabled by Neuraminidase-Resistant Glycopolymer-Coated Microbeads，ACS Appl. Mater. Interfaces 2021, 13, 39, 46260–46269，Publication Date:September 22, 2021
https://doi.org/10.1021/acsami.1c11561 （鏈霉親和素磁珠偶聯寡糖）

Abstract
Rapid diagnosis and vaccine development are critical to prevent the threat posed by viruses. However, rapid tests, such as colloidal gold assays, yield false-negative results due to the low quantities of viruses; moreover, conventional virus purification, including ultracentrifugation and nanofiltration, is multistep and time-consuming, which limits laboratory research and commercial development of viral vaccines. A rapid virus enrichment and purification technique will improve clinical diagnosis sensitivity and simplify vaccine production. Hence, we developed the surface-glycosylated microbeads (glycobeads) featuring chemically synthetic glycoclusters and reversible linkers to selectively capture the influenza virus. The surface plasmon resonance (SPR) evaluation indicated broad spectrum affinity of S-linked glycosides to various influenza viruses. The magnetic glycobeads were integrated into clinical rapid diagnosis, leading to a 30-fold lower limit of detection. Additionally, the captured viruses can be released under physiological conditions, delivering purified viruses with >50% recovery and without decreasing their native infectivity. Notably, this glycobead platform will facilitate the sensitive detection and continuous one-step purification of the target virus that contributes to future vaccine production.

摘要

快速診斷和疫苗開發對于預防病毒構成的威脅至關重要。然而，快速檢測，如膠體金檢測，由于病毒數量少，會產生假陰性結果。此外，常規病毒純化，包括超速離心和納濾，是多步驟且耗時的，這限制了病毒疫苗的實驗室研究和商業開發?？焖俨《靖患图兓夹g將提高臨床診斷靈敏度并簡化疫苗生產。因此，我們開發了具有化學合成糖簇和可逆接頭的表面糖基化微珠（糖磁珠），以選擇性捕獲流感病毒。表面等離子共振 (SPR) 評估表明 S-連接糖苷對各種流感病毒具有廣譜親和力。磁性糖珠被整合到臨床快速診斷中，導致檢測下限降低 30 倍。此外，捕獲的病毒可以在生理條件下釋放，以 > 50% 的回收率提供純化的病毒，并且不會降低其天然感染性。值得注意的是，該糖珠平臺將有助于對有助于未來疫苗生產的目標病毒進行靈敏檢測和連續一步純化。