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15805933710
地址：廈門市集美區集美大道1300號
電話：15805933710
聯系人：許先生
QQ：1974707632
微信：15805933710
郵件：purimag@139.com
■ 產品描述
PuriMag G-streptavidin鏈霉親和素磁珠通過鏈霉親和素和生物素之間的高親和力作用，可以綁定任何生物素標記的分子，如抗體、蛋白質、多肽、DNA等。粒子具有較大的比表面積和很高的捕獲效率。PuriMag G-streptavidin-biotinbiomolecule復合物通過磁分離易于從溶液中分離，因此提供了一個快速、簡潔的磁性納米粒子標記的生物分子的方法。鏈霉親和素磁珠可用于各種下游分離過程：蛋白純化、細胞分離、免疫及分子檢測。鏈霉親和素-生物素（SA-Biotin） 系統具有極高的結合親和力（K =10^-15） ， 在生物領域具有廣泛的應用。本產品采用超順磁性微球，粒徑均一、比表面積大、形貌規整，有利于方便、快捷、高效地捕獲目標分子以及實現磁性分離。本產品可配套自動化設備進行高通量操作。
■ 產品信息
尺寸: 200 nm
濃度: 10 mg/ml
配基: Streptavidin 鏈霉親和素
磁性: 超順磁
包覆材料： GMA
磁分離時間<30S
儲存：Store at 2-8°C（up to 6 months）in Storage Buffer (25 mM tris-HCl, 0.15 M NaCl, 0.05% tween20, 0.09% NaN3, pH 7.2). Do not freeze the reagent.
■ 結合能力
>50 μg Streptavidin/mg beads;
>4000 pmol free biotin/mg beads;
>300 pmol biotin-oligonucleotide/mg beads;
>20 pmol biotin-dsDNA fragments/mg beads;
本產品可完全替代市場主流鏈霉親和素磁珠如GE Sera-Mag? Magnetic Beads Streptavidin、Pierce Streptavidin Magnetic Beads、Dynabeads? MyOne? 鏈酶親和素、JSR的Streptavidin MS150等產品，表現為等同甚至更優的效果。
■質量控制
結合能力：每批鏈霉親和素磁珠均用游離生物素測試保證的最小結合能力。
蛋白質的非特異性結合：將2 mg SA磁性顆粒與0.2 mg IgG在200 μl的50 mM磷酸鹽緩沖液（0.15M NaCl，pH7.2）中溫育1小時（15-25℃）。用2×200 ul溫育緩沖液洗滌后，測上清液中的蛋白質濃度（A280）。未檢測到非特定的結合。
無DNA酶活性：將1 μg的DNA底物于37℃下在含有1mg的 SA磁珠的100ml緩沖液M （10mM Tris-HCl，10mM MgCl2，50mM NaCl，1mM DTT，pH7.5）中溫育4小時。未檢測到DNase活性。
無RNA酶活性：將5ug MS2 RNA在37℃下在100ml 10mM Tris-HCl，pH7.5和1mg SA磁性顆粒中溫育4小時。未檢測到RNase活性。
無蛋白酶活性：將10mg的 SA磁珠與casein-resorufin在200μl的0.2M Tris-HCl（20mM CaCl2，pH 7.8）中于37℃溫育30分鐘。 TCA沉淀后，蛋白酶活性與上清液中574nm處的吸光度相關。沒有檢測到蛋白酶活性。
■ 如何使用鏈霉親和素磁珠
鏈霉親和素磁珠PuriMag G-streptavidin可以用于捕獲生物素biotin標記的抗體、蛋白質、多肽、DNA等，可分為直接捕獲與間接捕獲兩種方式。如下所示：
■ 鏈霉親和素磁珠應用示例
例1. 生物素標記的核酸與SA磁珠的結合
例2. 鏈霉親和素磁珠固定化生物素化抗體用于化學發光
例3. 鏈霉親和素磁珠研究DNA/RNA與蛋白互作
鏈霉親和素磁珠詳細實驗方案見產品說明書，產品說明書請向客服索取。
■ 部分引用該磁珠文獻
1. X. Li, C. Zhao, Y. Liu, Y. Li, F. Lian, D. Wang, Y. Zhang, J. Wang, X. Song, J. Li, Y. Yang, K. Xu, Fluorescence signal amplification assay for the detection of B. melitensis 16M, based on peptide-mediated magnetic separation technology and a AuNP-mediated bio-barcode assembled by quantum dot technology, Analyst (2019), 8 （鏈霉親和素磁珠PuriMag G-Strep偶聯生物素修飾多肽檢測致病菌B. melitensis 16M）
2. Yuxi Wei, Yue Wang, Jiawei Wang, Xiaolin Yang, Honglan Qi, Qiang Gao, Chengxiao Zhang . Homogeneous electrogenerated chemiluminescence immunoassay for the detection of biomarkers by magnetic preconcentration on a magnetic electrode, Analytical and Bioanalytical Chemistry，July 2019, Volume 411, Issue 18, pp 4203–4211（鏈霉親和素磁珠PuriMag G-Strep/ Homo-Strep偶聯生物素修飾抗體，免疫電化學熒光檢測生物標志物）
3. Chuchu Wang Xiaoyan Du Tiantian Xie Hao Li. Label‐ and modification‐free‐based in situ selection of bovine serum albumin specific aptamer, journal of separation Science. First published: 24 September 2019 （鏈霉親和素磁珠PuriMag G-Strep用于適配體篩選）
4. Hao Kong, Wei-wen Liu, Wei Zhang, Qiang Zhang, Cun-huai Wang, Muhammad Idrees Khan, Yu-xing Wang*, Liu-yin Fan*, and Cheng-xi Cao*. Facile, Rapid, and Low-Cost Electrophoresis Titration of Thrombin by Aptamer-Linked Magnetic Nanoparticles and a Redox Boundary Chip, ACS Appl. Mater. Interfaces, 2019, 11, 33, 29549–29556（鏈霉親和素磁珠PuriMag G-Strep用于DNA適配體偶聯）
5. Xiao-Li Chen, Hao Zheng, Wen-Ge Li, You-Hong Zhong, Xiao-Ping Chen, Jin-Xing Lu. Direct blood culturing of Candida spp. on solid medium was achieved by a rapid enrichment method with magnetic beads coated with rhMBL, Journal of Clinical Microbiology, 2020（鏈霉親和素磁珠PuriMag Homo-Strep 純化抗體）
6. Yang Feng , Neng-Bin Xie , Wan-Bing Tao , Jiang-Hui Ding , Xue-Jiao You , Cheng-Jie Ma , Xiaoxue Zhang , Chengqi Yi , Xiang Zhou , Bi-Feng Yuan , and Yu-Qi Feng. Transformation of 5-Carboxylcytosine to Cytosine Through C–C Bond Cleavage in Human Cells Constitutes a Novel Pathway for DNA Demethylation, CCS Chem. 2020, 2, 994–1008 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾DNA)
7. Haiping Wu, Min Zhao, Jia Li, Xiaoyan Zhou, Tiantian Yang, Dezhang Zhao, Ping Liu, Huangxian Ju, Wei Cheng*, and Shijia Ding*, Novel Protease-Free Long-Lasting Chemiluminescence System Based on the Dox-ABEI Chimeric Magnetic DNA Hydrogel for Ultrasensitive Immunoassay, ACS Applied Materials & Interfaces 2020, 12, 42, 47270-47277 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾DNA)
8. Luyao Shen, Keke Jia, Tao Bing*, Zhibao Zhang, Xiaoxiao Zhen, Xiangjun Liu, Nan Zhang, and Dihua Shangguan*. Detection of Circulating Tumor-Related Materials by Aptamer Capturing and Endogenous Enzyme-Signal Amplification, Anal. Chem. 2020, 92, 7, 5370–5378 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾適配體)
9. Shanshan Yu, Limei Ai, Wei Wei, circRNA circ-MYBL2 is a novel tumor suppressor and potential biomarker in multiple myeloma, Human Cell, volume 34, pages219–228(2021) (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾RNA pull down)
10. Xi Rao Lingling Lai Xiaopeng Li Liang Wang Ai Li Qian Yang. N6‐methyladenosine modification of circular RNA circ‐ARL3 facilitates Hepatitis B virus‐associated hepatocellular carcinoma via sponging miR‐1305, IUBMB LIFE, Volume73, Issue2, February 2021, Pages 408-417(鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾RNA pull down)
11. Hongwei Hu , Yujing Ding , Zihan Gao , Hao Li. S1 nuclease digestion-based rational truncation of PD-L1 aptamer and establishment of a signal dual amplification aptasensor, Sensors and Actuators B Chemical, 2021, 331:129442, DOI:10.1016/j.snb.2021.129442(鏈霉親和素磁珠偶聯抑制因子篩選適配體)
12. Qing An, Zipeng Zhou, Yi Xie, Yu Sun, Haixiang Zhang & Yang Cao (2021) Knockdown of long non-coding RNA NEAT1 relieves the inflammatory response of spinal cord injury through targeting miR-211-5p/MAPK1 axis, Bioengineered, 12:1, 2702-2712, DOI:10.1080/21655979.2021.1930925 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾RNA pull down)
13. Dongyue Hu, Shusen Xiao, Qiaqia Guo, Rongrong Yue, Demin Geng and Debin Ji, Luminescence method for detection of aflatoxin B1 using ATP-releasing nucleotides, DOI: 10.1039/D1RA03870B (Paper) RSC Adv., 2021, 11, 24027-24031 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾適配體)
